MiR-483-3p exacerbates pediatric pneumonia by suppressing IGF1 expression in alveolar macrophage


 Purpose: To investigate the action of miR-483-3p in pediatric pneumonia and identify potential biomarkers.
 Methods: Bronchoalveolar lavage was collected from 38 patients 25 healthy children. The expression determined using qRT-PCR. Alveolar macrophages children were cultured used for functional assays. Transwell assay conducted to evaluate macrophage cell migration. Cell viability apoptosis evaluated lipopolysaccharide (LPS)-induced human pulmonary alveolar epithelial cells (HPAEpiCs) by CCK8 (cell counting kit - 8) or flow cytometry, respectively.
 Results: MiR-483-3p significantly elevated bronchoalveolar patients, when compared (p < 0.001). MiR-483-3p, which targets insulin-like growth factor 1 (IGF1), decreased mRNA protein IGF1 reduced motility macrophages. counteracted LPS- induced decrease increase HPAEpiCs. Conditioned medium transfected with inhibitor increased cellular LPS-induced However, conditioned mimics apoptosis.
 Conclusion: negatively regulates promote progression pneumonia, providing a therapeutic target pneumonia.